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   常識及應用

[ 2010/9/1 11:26:24 ]

 

 

Determination of silica activity

It usually use three kinds of dyes to determine thin layer chromatography.European pharmacopoeia recorded in 1969 that the benzene of 0.01% Dimethy-yello. P-Di methylaminazobenzene, Sudan Ⅲ, Indophenol blue 4-Tapnthoquinone-4-dimethyl aminoaniline, each part of component is 10ul.Then bit benzene solvent on thin layer of silica gel G or silica gel H, using benzene as developing agent, expand 10cm (about 20 minutes), three kinds of dyes must be separated distinctly. The indophenol blue spot is near the starting line, dimethy yellow spots is in the middle of thin layer plate, it is considered to meet the requirements when between Sudan spot and dimethyl yellow spot.

 



Column Chromatography Silica Gel

Thin Layer Chromatography Silica Gel Plate

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